| Oligonucleotide D1 (100 μM) | 1.5 μL |
| T4 polynucleotide kinase buffer (10X) | 10 μL |
| T4 polynucleotide kinase (10 U/μL) | 4 μL |
| [γ-32P]ATP, 10 mCi/mL | 10 μL |
| H2O | 74.5 μL |
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