Table I.
Summary of all growth delay experiments with 4T07, HCT116, FaDu, SKOV-3 and PC-3. A single dose of 6mg/kg of LTSL-DOX i.v. and 1-h long HT treatment at 42° C were used in all experiments, with the exception of 4T07, where 7 mg/kg was used. The table lists results of 1–3 independent experiments with each tumour type. Data from the experiment in Figures 1A and 1B are shown in bold. Median growth time (MGT) is the median number of days required to reach five times initial tumour volume, or 60 days after treatment. Growth delay (GD) is defined as (MGT of treatment group) minus (MGT of control group at the same temperature), with the exception of the HT group, where GD is defined as the difference in MGT between HT and the unheated control groups. Response fraction (RF) is the ratio of tumour regressions (complete and incomplete) to the number of animals in all experiments of that tumour type and treatment, where an incomplete regression is a reduction >30% of starting tumour volume. The number of complete regressions, if they are present, is listed after RF (for example, for the HT group and SKOV-3, 2/10, 1 means two out often responses, with 1 complete regression). n = 8–10 animals for all treatment groups of each tumour, in all experiments. ◈ and ◈◈ indicate a significant difference with p < 0.05 and p < 0.01, respectively, from MGT of a control group treated at the same temperature (Mantel-Haenszel log-rank test), and therefore no Bonferroni correction was performed here. p>0.05 is denoted by X.
| 4T07 |
HCT116 |
FaDu |
SKOV-3 |
PC-3 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| RF | RF | RF | RF | RF | |||||||
| Control | MGT (days) | 7.5 | 0/20 | 8.8 | 0/20 | 12.1 | 0/10 | 17.2 | 2/10 | 20.9 | 0/18 |
| 8.7 | 8.2 | 22.6 | |||||||||
| HT | MGT (days) | 8.1 | 0/18 | 11.0 | 0/20 | 14.9 | 0/10 | 17.4 | 2/10, 1 | 23.0 | 3/20 |
| 12.2 | 9.9 | 34.6 | |||||||||
| GD (days) | 0.6 | 2.2 | 2.7 | 0.2 | 2.2 | ||||||
| 3.5 | 1.6 | 11.6 | |||||||||
| LTSL-DOX | MGT (days) | 10.7 ◈ | 0/15 | 10.6X | 0/20 | 16.3 ◈ ◈ | 0/10 | 22.4 ◈ | 2/10, 2 | 24.0X | 0/19 |
| 10◈◈ | 8.8X | 30.1◈◈ | |||||||||
| GD (days) | 3.2 | 1.8 | 4.2 | 5.2 | 3.2 | ||||||
| 1.3 | 0.6 | 7.5 | |||||||||
| HT-LTSL-DOX | MGT (days) | 19.8 ◈ ◈ | 0/14 | 28.1 ◈ ◈ | 1/20, 1 | 41.8 ◈ ◈ | 1/10 | 51.9 ◈ ◈ | 7/10, 3 | 60 ◈ ◈ | 15/20, 4 |
| 16.2◈ | 20.8X | 60◈ | |||||||||
| GD (days) | 11.7 | 17.1 | 26.9 | 34.4 | 37.0 | ||||||
| 4.0 | 10.8 | 25.4 | |||||||||