Table 5.

List of primers for the seven proposed DNA barcoding markers.

Marker	Primer sequence	Amplicon size (Lemna minor)	Ta Optimum (Lemna minor)
psbK-psbI	Forward: 5'-TTAGCATTTGTTTGGCAAG-3';	544 bp	51°C
	Reverse: 5'- AAAGTTTGAGAGTAAGCAT -3'
trnH-psbA	Forward: 5'-GTTATGCACGAACGTAATGCTC-3';	300 bp	55°C
	Reverse: 5'- CGCGCGTGGTGGATTCACAATCC-3'
matK	Forward: 5'-CGTACTGTACTTTTATGTTTACGAG-3';	862 bp	55°C
	Reverse: 5'- ATCCGGTCCATCTAGAAATATTGGTTC -3'
atpF-atpH	Forward: 5'-ACTCGCACACACTCCCTTTCC-3';	675 bp	53°C
	Reverse: 5'- GCTTTTATGGAAGCTTTAACAAT -3'
rpoB	Forward: 5'-ATGCAGCGTCAAGCAGTTCC-3';	406 bp	55°C
	Reverse: 5'- TCGGATGTGAAAAGAAGTATA -3'
rpoC1	Forward: 5'-GGAAAAGAGGGAAGATTCCG-3';	509 bp	56°C
	Reverse: 5'- CAATTAGCATATCTTGAGTTGG -3'
rbcL	Forward: 5'-GTAAAATCAAGTCCACCACG-3';	580 bp	56°C
	Reverse: 5'-ATGTCACCACAAACAGAGACTAAAGC -3'