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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Brain Res. 2010 Sep 21;1358:71–80. doi: 10.1016/j.brainres.2010.08.080

Figure 1.

Figure 1

Confocal fluoroescence micrographs in the striatum showing GPR177 and MOR-opioid receptor immunoreactivities. A-B. GPR177 was detected using a rhodamine isothiocyanate-conjugated secondary antibody (red) and MOR was detected a fluorescein isothiocyanate-conjugated secondary antibody (green). C. Merged image. D-E. Photomicrographs of tissue sections that were processed in parallel without the primary antibodies. F. Schematic diagram adapted from the rat brain atlas of Paxinos and Watson (Paxinos and Watson, 1997) depicting the region sampled (trapezoid) for immunohistochemical analysis. Arrowheads point to individual GPR177-labeled perikarya that contain MOR. The single arrow points to a perikaryon containing GPR177 immunoreactivity only. In panels A and F, arrows indicate dorsal (D) and medial (M) orientation of the tissue section. ac, anterior commissure; cc, corpus callosum; LV, lateral ventricle; ox, optic chiasm. Scale bars, 100 μm.