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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Virus Res. 2010 Aug 26;153(2):269–276. doi: 10.1016/j.virusres.2010.08.018

Figure 3. The target of PYR on the HIV-1 life cycle. (A) PYR does not activate HIV-1 LTR promoter activity.

Figure 3

MT-2 cells were transiently transfected with HIV-1 LTR-Luciferase reporter gene (p461) or co-transfected with tat expression plasmid. Cells were cultured in the presence or absence of PYR (10 μM) for 48 hrs and then luciferase activity was measured. The activity was normalized by total cellular protein. The schematic diagram of the plasmid construct containing the HIV-1 LTR-Luciferase reporter gene (p461) is shown.

(B) PYR has no effect on viral budding. HIV infection was performed as above. Intracellular p24 from whole cell extract and extracellular p24 from the cell supernatant were measured by p24 antigen capture assay. Results are representative of three independent experiments.

(C) PYR increases HIV-1 protein expression in MT-2 cells. Whole MT-2 cell extract from days 2, 4, and 7 after HIV-1 infection were prepared with RIPA buffer, and 20 μg of total protein was analyzed by Western blotting. Membrane was probed with HIV-infected patient plasma. The membrane was stripped and subsequently reprobed with anti-β-actin as internal control for equal loading (lower panel).