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. 2010 Oct 18;5(10):e13130. doi: 10.1371/journal.pone.0013130

Figure 1. NV replication induces Golgi fragmentation.

Figure 1

Viral RNA, purified from the stool of a human volunteer infected with Norwalk virus, was transfected into Huh7 cells grown on coverslips. At 24 hours post-transfection (hpt), cells were fixed and stained for the viral protein VP1 (Alexa 488; green fluorescence) and either the cis (A) or trans (B) Golgi with antibody against GM130 or golgin-97 (Alexa 594; red fluorescence), respectively. Nuclei were stained with DAPI (blue fluorescence) and imaged by deconvolution microscopy. * indicate cells with disassembled Golgi.