Figure 4. Dynamics of intracellular Ca²⁺ in RGC apoptosis.

(A) Illustrative examples of intracellular Ca²⁺ dynamics in two individual differentiated RGC-5 cells during apoptosis under 100 mmHg pressure. One cell shows single Ca²⁺ peak (blue line) and the other shows multiple peaks (red line). Note that the Ca²⁺ elevation shows two-stage process of gradual increase over about one to two hours followed by a short transient peak of about 0.5 hour duration. (B) The fraction of cells showing Ca²⁺ elevation with a single peak, with multiple peaks, and no Ca²⁺ elevation. Values are mean ± standard deviation from 5 independent experiments.