Figure 4. FISH painting of BAC-containing markers of the 5 chromosomes of Brachypodium (Bd).
A. BAC pool of Bd1 (green) with ABR1-26-H1 – anchor for 1L (red). B. BAC pool of Bd2 (green) with ABR1-41-E10 – anchor for 2S (red). C. BAC pool of Bd3 (green) with ABR5-33-F12– anchor for 3S (red). D. BAC pool of Bd4 (green) with ABR5-33-F2 – anchor for 4S (red). E. BAC pool of Bd5 (green) with 25S rDNA – anchor for 5S (red) indicated by the white arrows. The details of each BAC pool are described in Table S2. All bars: 5 µm.