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. 2009 Dec;29(12):817–824. doi: 10.1089/jir.2009.0034

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 2. — Ip-10 is transcribed with delayed kinetics due to synthesis of an interferon (IFN)-stimulated regulatory element (ISRE)-binding factor. (A) Wild-type MEFs were treated with 1,000 IU/mL of murine IFN-γ for various times and ip-10 and gapdh mRNAs were measured by the Northern method. (B) The rate of ip-10 transcription in wild-type MEFs in response to IFN-γ measured by qPCR, using primers that specifically amplify primary ip-10 RNA transcripts. (C) Electrophoretic mobility shift assay (EMSA) analysis of nuclear lysates from IFN-γ-treated wild-type MEFs, using an oligonucleotide probe representing the ISRE from the ip-10 promoter.