Table 3. Energy-dependence of the uptake of L-[3H]carnitine by HCLE or HCjE cells at pH 7.5 and 37 °C.
|
|
Relative uptake (% of control) |
|
|---|---|---|
| Compound | HCLE | HCjE |
| Sodium azide |
80.4±1.8 |
79.2±1.3 |
| Dinitrophenol |
69.1±4.7 |
63.1±7.6 |
| Iodoacetic acid | 27.2±6.3 | 27.8±3.3 |
HCLE or HCjE cells were pre-incubated for 30 min in uptake buffer containing 10 mM of sodium azide, dinitrophenol or iodoacetic acid; L-[3H]carnitine (24 nM) was then added and the incubation continued for 30 min. Each value is the percentage uptake of L-[3H]carnitine in medium containing sodium azide, dinitrophenol, or iodoacetic acid compared with that in control medium without these compounds. Values are the mean±SD of results from four experiments.