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. 2010 Oct 18;5(10):e13412. doi: 10.1371/journal.pone.0013412

Figure 3. p130Cas SD signaling stimulates leading edge protrusion.

Figure 3

Monolayer scratch wounds were generated and DIC images of cells migrating at the wound edge were captured every 3 seconds for 15 minutes and used to evaluate leading edge dynamics using kymography. (A) Sample kymographs representative of mean protrusion values. Vertical scale bar is 5 µm, horizontal scale bar is 2 minutes. (B) Rates of protrusion representing the mean of 60–90 individual cells for each cell type. Leading edge protrusion velocity was quantified by measuring the distance and duration of individual protrusions from 3 kymographs per cell. Bars indicate s.e.m. Significance values were determined by one-way ANOVA followed by Tukey-Kramer post hoc testing; n.s. (not significant), ***p<0.001.