Figure 6. SBD-mediated SD signaling is implicated in stimulating both FA assembly and disassembly rates.

Apparent rate constants of FA assembly (r_rise) and disassembly (r_decay) were determined by fitting FA assembly to a logistic function and the final FA disassembly to exponential decay. (A) Two examples of curve fits to FA intensity data (grey line) for assembly (purple dotted line) and disassembly (red dotted line) representative of the mean r_rise and r_decay are shown for each cell type. The graphs are normalized so that the modeled logistic function plateau equals 100. (B and C) Mean r_rise and r_decay values for each cell type are shown. Data represent the mean of 45–100 FAs from 5 cells. Bars indicate s.e.m. Significance values were determined by one-way ANOVA followed by Tukey-Kramer post hoc testing; n.s. (not significant), where all other comparisons are significant at ***p<0.001. (D and E) Histogram distributions of the r_rise and r_decay values for each cell type are shown in intervals of 0.25×10⁻² Hz. In panel D, note that the majority of WT r_rise values are greater than 1.00×10⁻² Hz while for all other cell types the majority of r_rise values are less than 1.00×10⁻² Hz and have similar distributions. In panel E, note that the majority of WT r_decay values are greater than 0.50×10⁻² Hz while for all other cell types the majority of r_rise values are less than 0.50×10⁻² Hz and have similar distributions.