Table 1. Primers for amplification of PITX3 from genomic DNA.
Primer | Sequence | Tm (°C) | Size (bp) |
---|---|---|---|
PITX3-Ex1-L1 |
CTGCCATAAAGTGAATGGGCGC |
49–60 |
278 |
PITX3-Ex1-R1 |
TCCAAGGTCCAGCAATAGCTCCTC |
|
|
PITX3-Ex2-L1 |
CCATTACCCTGGTCTGTGTCTTCTCTC |
56–60 |
221 |
PITX3-Ex2-R1 |
CCCGCACTGGGGATGAAGC |
|
|
PITX3-Ex3-L1 |
TGGGCGCTCTGTGACCTGC |
56–60 |
273 |
PITX3-Ex3-R1 |
CGCGGGTGCGAGTCGC |
|
|
PITX3-Ex4-L1 |
CCTTCAGCCGCTGGGACC |
63–67 |
1000 |
PITX3-Ex4-R1 | TCAAGCGCAACTTTGAATCATCAC |