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. 2010 Oct 18;5(10):e13462. doi: 10.1371/journal.pone.0013462

Figure 6. Expression of 14-3-3ζ K49E leads to a specific increase in L1-mediated neurite outgrowth.

Figure 6

Hippocampal neurons prepared from embryonic rat hippocampus were transfected by nucleofection with an expression plasmid for EGFP together with plasmids encoding either empty vector (“Mock”), wild-type 14-3-3ζ (“WT”) or 14-3-3ζ K49E (“K49E”). Subsequently, cells from each transfection were plated onto wells coated with PLL in combination with Fc (“Fc substrate”) or L1-Fc (“L1-Fc substrate”). After incubation for 24 h at 37°C, cells were fixed. A. The images show transfected neurons in three transfected groups grown either on Fc or L1-Fc substrates. B. Length of the longest neurite per cell was measured for neurons in the same groups as in (A.). Values are expressed as mean ±SEM. Per substrate and expressed protein, neurons from 3 transfections, 3 wells per transfection, ∼30 cells per well were analyzed. Two-way ANOVA with repeated measures (for substrate) revealed significant effects of transfection (p<0.05), substrate (p<0.001) and interaction between transfection and substrate (p<0.05). **p<0.01, ***p<0.001, statistically significant differences vs. 14-3-3 K49E; #p<0.05, ##p<0.01, ###p<0.001, statistically significant differences between neurons grown on Fc and L1-Fc (Bonferroni's post-hoc test, n = 3 transfections).