FIG. 1.

Schematic of the Transcreener ADP assay principle. ADP produced during transfer of phosphate to acceptor substrate is detected using a competitive FP immunoassay. Unlike antibody-bound tracer (high mP), displaced tracer (low mP) spins rapidly in solution, resulting in a lower proportion of emitted polarized light. Because ADP detection relies on detection of the invariant reaction product, the same detection reagents can be used for any kinase and any substrate or any ADP-producing enzyme.