Skip to main content
. 2009 Feb;7(1):56–67. doi: 10.1089/adt.2008.175

FIG. 6.

FIG. 6.

Stability of the FP signal and the ADP detection mixture over time. (A) Equilibration of FP signals for ATP/ADP standards representing different percentages of ATP conversions (10 μM ATP/ADP standard curve). Plates were incubated at room temperature and read at 4-min intervals for 1 h after addition of ADP Detection Mixture 2. Error bars represent standard deviations of the mean (n = 16). (B) Long-term signal stability of the ATP/ADP standard samples. The 10 μM ATP/ADP standard curve was prepared with ADP Detection Mixture 2, and the FP signal was recorded at 0 h (○), 1 h (•), 4 h (▾), 8 h (♦), and 24 h (▴). Error bars represent standard deviations of the mean (n = 24). (C) 10 μM ATP/ADP standard curves prepared with stock ADP detection mixtures. ADP detection mixtures were prepared and stored for 21 days at the designated temperatures prior to adding to freshly prepared ATP/ADP standards: Day 0 control (♦), −80°C (u), −20°C (▵), 4°C (▿), 25°C (◊), and 37°C (○). The Day 0 control (standard curve) was prepared with fresh ADP Detection Mixture 2. Error bars represent standard deviations of the mean (n = 4).