FIG. 7.

Control compound screen for assessing assay interference. Test compounds at 10 μM (1% dimethyl sulfoxide) were incubated with 10 mM ATP and a mixture of 1 μM ADP and 9 μM ATP (to represent 10% ATP conversion) in the CK reaction buffer: 0% ATP conversion (•), 10% ATP conversion (▴), 0% ATP conversion control (○), 10% ATP conversion control (▵), and 100% ATP conversion control (u). An equal volume of ADP Detection Mixture 2 (20 mM HEPES [pH 7.5], 40 mM EDTA, 0.02% Brij-35, 4 nM ADP-Alexa Fluor633 tracer, and 15 μg/ml Ab2) was then added to each well. The Z′ value for the 10% ATP conversion control was 0.89 (n = 384 from 24 plates). Compounds were tested in triplicate. Controls shown on the graph are from one plate (n = 16).