Table 2.
Specificity and Sensitivity of a qRT-PCR Assay That Distinguishes all Human IFN-α Subtypes
|
Template subtype added (106 copies) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 or 13 | 2 | 4 | 5 | 6 | 7 | 8 | 10 | 14 | 16 | 17 | 21 | ||
| IFN-α subtype assay | 1 or 13 | 7.46 | |||||||||||
| 2 | 6.94 | ||||||||||||
| 4 | 6.54 | ||||||||||||
| 5 | 6.93 | ||||||||||||
| 6 | 6.17 | 28.34 | |||||||||||
| 7 | 26.46 | 7.51 | 28.19 | ||||||||||
| 8 | 6.93 | ||||||||||||
| 10 | 6.62 | ||||||||||||
| 14 | 23.87 | 23.71 | 22.98 | 7.73 | 25.13 | ||||||||
| 16 | 6.97 | 26.47 | |||||||||||
| 17 | 9.42 | ||||||||||||
| 21 | 8.64 | ||||||||||||
Grey-filled cells: specific amplicon signal (Ct value).
Bold-italics: Value is below that calculated for one gene copy.
Blank: Value below threshold of detection (40 Ct).