Table 3.
IFN-α Protein (ng/mL) Secretion by Human pDC in Response to TLR Agonists
|
Treatmenta |
No stimulus |
A-class CpG |
C-class CpG (10101) |
C-class CpG (10109) |
Flu virus Liveb |
Flu virus inactive |
R837 |
|||
|---|---|---|---|---|---|---|---|---|---|---|
| IL-10c | − | − | + | − | − | − | − | + | − | + |
| Donor | ||||||||||
| 1 | 0.021 | 2,000 | 140 | 320 | 310 | 1,400 | ||||
| 2 | 0.004 | 810 | 39 | 220 | 180 | 390 | ||||
| 3 | 25 | 8.1 | 28 | 11 | ||||||
| 4 | 64 | 5.1 | 33 | 10 | ||||||
| 5 | 76 | 19 | 87 | 7.2 | 3.5 | 0.7 | ||||
| 6 | 39 | 8.3 | 0.7 | 0.2 | ||||||
| 7 | 100 | 9.6 | 0.7 | 0.2 | ||||||
| 8 | 79 | 78 | 100 | 310 | 520 | |||||
| 9 | 750 | 170 | 190 | 380 | 390 | |||||
a
Supernatants were collected from individual cultures at 16 h poststimulation except for donors 8 and 9. For those cultures pDC were stimulated for 6, 12, and 18 h. The 18-h data are provided here for comparison and results from all time points are shown in Figure 4A. bFor donor 1, live and inactivated influenza virus was applied to cultures at an MOI of 0.5 and 5. Shown are data for MOI of 5. cpDC cultures were treated with (+) or without (−) 5 ng/mL of recombinant human IL-10 upon stimulation.