Figure 2. Hip4 is stably associated with the subunits of the HIRA complex.

(A) Whole cell extracts (WCE) or whole cell extracts partially purified with IgG-coupled Dynal beads (IP) were analysed by western blotting with anti-Pk and peroxidase anti-peroxidase (α-PAP) antibodies. The strains used are indicated below the lane. (B) As for (A). (C) As for A except that anti-FLAG antibody was used in place of anti-Pk antibody. Experiments were performed at least twice and a representative example is shown.