Figure 3. Phenotypes associated with deletion of hip4 ⁺.

(A) hip4Δ cells are temperature sensitive. The indicated strains were grown to exponential phase, subjected to five-fold serial dilutions and spotted onto YE5S agar plates and incubated at 30°C or 37°C. (B) Comparison of the morphology of wild type, hip1Δ, slm9Δ, hip3Δ and hip4Δ cells. (C) Comparison of the morphology of wild type cells transformed with empty vector (pRep41), hip4Δ cells transformed with empty vector and hip4Δ cells transformed with a plasmid expressing hip4⁺ (pRep41-Hip4) from the nmt promoter. (D) hip4Δ cells are hypersensitive to spindle damage. The indicated strains were grown to exponential phase, subjected to five-fold serial dilutions and spotted onto YE5S agar or YE5S supplemented with thiabendazole (TBZ) at 15 µg/ml. Plates were incubated at 30°C. Experiments were performed at least three times and a representative example is shown.