Table 2.
Experimental design of cardio physiological, pulmonary and systemic response..
| Instillation | ||||||||
|---|---|---|---|---|---|---|---|---|
| Adaptatiion | Baseline | Post exposure | ||||||
| Cardiovascular response | Day -2 | Day-1 | Day 0 | Night 1 | Night 2 | Night 3 | Night 4 | Night 5 |
| Acclimatization | √ | √ | √ | √ | √ | √ | ||
| Telemetry (n = 5)* | No change | BP | BP | BP | BP | BP | BP | |
| HR | HR | HR | HR | HR | HR | |||
| ambient particle-mediated pulmonary and systemic response | ||||||||
| Pulmonary resposne | Day 1 (24 h; Group A) | Day 3 (72 h; Group B) | ||||||
| Dose/rat | 250 μg | 500 μg | 1000 μg | 500 μg | 1000 μg | |||
| BALF (n = 6) | √ | √ | √ | √ | √ | |||
| Lung tissue (n = 6) | √ | √ | √ | √ | √ | |||
| Pulmonary histopathology (n = 2) | √ | √ | √ | √ | √ | |||
| Systemic response | ||||||||
| Blood (n = 8) | √ | √ | √ | √ | √ | |||
| Cardiac tissue (n = 6) | √ | √ | √ | √ | √ | |||
| Cardiac histopathology (n = 2) | √ | √ | √ | √ | √ | |||
*Number of exposed animals is given. A similar number of animals were intratrachealy instilled with pyrogene-free distilled water and served as controls or sham. Blood : blood collected from retro arbitral sinus and abdominal aorta.. BP and HR: blood pressure and heart rate were the two primary parameters measured for the cardiovascular response.