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Enrichment of chloroplast DNA from karaka. The relative quantity of chloroplast DNA was determined in preparations of genomic DNA (gDNA), chloroplast-enriched DNA (cpDNA) and RCA amplified chloroplast-enriched cpDNA (RCA). Quantitative PCR amplification of the plastid encoded psbB gene was compared to amplification of nuclear 18S rRNA. The mean of four technical replicates ± SE has been reported as fold-differences relative to gDNA.
