Table 3.
Plasmids used in this study
| Plasmid | Genotype | Source, reference |
|---|---|---|
| pACLacI | pACYC184 derivative with lacIq; CmR | This study |
| pASK75 | vector, P/Otet, tetR, ColEI ori; ApR | [60] |
| pASKSurAa | surA gene in pASK75; ApR | [2] |
| pASKSurAN-Ctb | surAN-Ct fusion from pSurAN-Ct [2] in pASK75; ApR | This study |
| pASKssPpiD | surA signal sequence-his6-ppiD (codons 34-623) fusion in pASK75; ApR | This study |
| pASKssPpiDΔParv | pASKssPpiDΔ252-355; ApR | This study |
| pΩSurA | Ω::spec-PLlac-O1 surA in pUC18; ApR; SpecR | This study |
| pMP1 | skp gene region of E. coli MC1061 (corresponding to nucleotides 199495-201937 of the E. coli MG1655 genomec) in pSU18; CmR | Gross laboratory |
| pPLT13 | mini-F carrying lacIq; KanR | [61] |
| pPpiD | ppiD gene and promoter of E. coli MC1061 (corresponding to nucleotides 460852-463020 of the E. coli MG1655 genomec) in pTrc99A (lacIq and Ptrc sequences deleted); ApR | This study |
| pPpiDI350A | pPpiD carrying mutation I350 to A; ApR | This study |
| pPpiDG347A | pPpiD carrying mutation G347 to A; ApR | This study |
| pPpiDΔ69-201 | pPpiDΔ69-201; ApR | This study |
| pPpiDΔParv | pPpiDΔ252-355; ApR | This study |
| pPpiD(ΔTM) | surA signal sequence-his6-ppiD (codons 34-623) fusion in pPpiD; ApR | This study |
| pPpiDfs601 | pPpiD with a frameshift mutation in ppiD that generates a stop at codon 173; ApR | This study |
| pQE60 | C-His6 fusion vector, PT5/Olac, ColEI ori; ApR | Qiagen |
| pSkp | skp gene and skp promoter of E. coli MC1061 (corresponding to nucleotides 200073-201801 of the E. coli MG1655 genomec) in pQE60 (PT5/Olac deleted); ApR | This study |
| pTrc99a | Expression vector, Ptrc, ColEI ori; ApR | Amersham Pharmacia |