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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: Am J Physiol Cell Physiol. 2008 Jan 16;294(3):C786–C796. doi: 10.1152/ajpcell.00457.2007

Figure 4. IL-8 and liver cyst fluid promote HMEC-1 cell proliferation.

Figure 4

(A) Treatment of HMEC-1 cells with increasing doses of IL-8 for 24 hrs resulted in a significant increase in cell proliferation at 0.1 ng/ml and a maximal response at 100 ng/ml IL-8 when compared to unsupplemented control (Con) cells. From an analysis of variance, bars without a shared letter (e.g. A, B, C, D) are significantly different from each other. (B) Compared to unsupplemented HMEC-1 cells, cells treated with 10% human liver cyst fluid markedly induced cell proliferation. Pretreatment with 50 nM SB225002 inhibited the proliferative response in paired assays (*p<0.05 vehicle vs SB225002). Treatment with 10% human renal cyst fluid also induced HMEC-1 cell proliferation. From four studies, pretreatment with 50 nM SB225002 did not significantly block the proliferative effect of 10% human renal cyst fluid.