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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mech Dev. 2010 Jul 7;127(9-12):418–427. doi: 10.1016/j.mod.2010.07.001

Figure 3.

Figure 3

Impaired coronary artery maturation and disorganized myocardial vasculature in left ventricles of CHF1/Hey2 knockout mice. PECAM (a, b) and αSMA (c, d) immunostaining were performed for E18.5 embryonic hearts. The control left ventricles (a, c; n=7) generally form well-developed coronary arteries with a distinct circumferential αSMA positive smooth muscle layer (arrows). In contrast, mutant ventricles (n = 8) showed dysmorphic vessels with a partially disrupted smooth muscle layer (d, arrows, inset) within a disorganized myocardial vasculature (b, arrowheads). Scale bars = 200 μm.