Bone marrow progenitor cells were isolated from C57BL/6 mice (n = 4–5 mice), differentiated with GM-CSF for six days, and then stimulated with the indicated TLR agonist, individually or in combination. After 12 h, culture supernatants were collected, and cytokines levels were measured by ELISA. Samples were assayed in triplicate. Data represent the mean ± SEM from three experiments. *, statistically significant p≤0.05 (18.months vs. 2, 6, or 12 months).