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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Exp Gerontol. 2010 Jul 1;45(11):834–841. doi: 10.1016/j.exger.2010.06.007

Figure 4. PGE2 upregulates IL-23 production from aged DCs.

Figure 4

(A) PGE2 increases IL-23 production by aged DCs. Bone marrow progenitor cells were isolated from C57BL/6 mice (n = 5 mice), differentiated with GM-CSF for six days then cultured in media with or without 10 mM PGE2. After 1h, LPS+R848 was added for an additional 6 h. IL-23 levels in the culture supernatants were determined by ELISA. (B) Indomethacin decreases IL-23 production that is induced by LPS+R848 in aged DCs. DCs were isolated and grown with GMCSF for 6 days then treated with 10 μM Indomethacin, After 24 h, LPS+R848 was added for an additional 6 h. IL-23 levels in the culture supernatants were determined by ELISA. (C) EP2 and/or EP4 antagonist reduces IL-23 induction by LPS+R848. DCs were isolated and stimulated as above then treated with 50μM of EP2 antagonist (AH6809), EP4 antagonist (AH 23848), or EP2+EP4 antagonists. After 1 h, LPS+R848 was added for an additional 6 h, IL-23 levels in the culture supernatants were determined by ELISA. Samples were assayed in triplicate. Data represent the mean ± SEM from three experiments. *, statistically significant (p<0.05), 18 months vs. 2 months.