Fig. 2. DL3 and Bic did not induce apoptosis in LNCaP cells.

A. LNCaP-ARR₂PB-GFP cells were incubated for 72 hr in phenol-free medium supplemented with 5% charcoal-dextran stripped FBS, followed by treatment with 20 uM of DL3 or Bic in the absence or presence of 1 nM of DHT for 96 hr. The cultures were observed under a fluorescent microscope and recorded. B. LNCaP cells were incubated for 72 hr in phenol-free medium supplemented with 5% FBS, followed by treatment with various concentrations of DL3 in the absence or presence of 1 nM of DHT for 48 hr. Cellular caspase 3 level was revealed by immunoblotting with β-actin as loading control. Data shown were one representative experiment of 3.