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. Author manuscript; available in PMC: 2011 Dec 8.
Published in final edited form as: Cancer Lett. 2010 Aug 2;298(2):250–257. doi: 10.1016/j.canlet.2010.07.012

Fig. 3. DL3 and Bic induced G1 phase arrest in LNCaP cells.

Fig. 3

LNCaP and PC-3 cells in 6-well plates (5 × 105 and 2.5 × 105/well, respectively) were starved in serum-free medium for 24 hr, followed by stimulation with 10% FBS plus 1 nM DHT in the absence or presence of 10 or 20 uM of DL3 or Bic. The cells were sampled every 24 hr up to 72 hr for cell cycle analysis by FACS as detailed in Materials and Methods. Data shown were the cell cycle distribution pattern of cells treated for 48 hr. This is one representative experiment of 5.