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. Author manuscript; available in PMC: 2011 Dec 8.
Published in final edited form as: Cancer Lett. 2010 Aug 2;298(2):250–257. doi: 10.1016/j.canlet.2010.07.012

Fig. 4. Effects of DL3 and Bic on the expression of G1 phase-related cell cycle-regulating proteins.

Fig. 4

A. LNCaP and PC-3 cells were treated for 48 hr with 20 uM of DL3 or Bic. Cell lysates were prepared and analyzed by immunoblotting with β-actin as loading control. B and C. LNCaP and PC-3 cells were starved in serum-free medium for 24 hr and then treated for 24 hr with 10% FBS in the absence or presence of 20 uM of DL3 or Bic. Cell lystates were analyzed by western blotting with β-actin as loading control. D. LNCaP and PC-3 cells were starved in serum-free medium for 24 hr and then treated for 24 hr with 10% FBS in the absence or presence of 20 uM of DL3 or Bic. Cell lystates were immunoprecipitated with an antibody to Rb and analyzed by western blotting with antibodies against CDK substrates and pRb (ser807/811). Data shown is from one representative experiment of 3.