Table 1.
Differential effects of DL3 and Bic on cell cycle distribution in LNCaP and PC-3 cells
| LNCaP | PC-3 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time (hr) |
Phase of cell cycle |
FBS (0%) |
10%FBS | FBS 0% |
10% FBS | ||||||||
| DL3 (uM) | Bic (uM) | DL3 (uM) | Bic (uM) | ||||||||||
| 0 | 10 | 20 | 10 | 20 | 0 | 10 | 20 | 10 | 20 | ||||
| G1 | 75 | 65 | 70 | 73 | 68 | 70 | 56 | 43 | N | 47 | N | 45 | |
| 24 | S | 1 | 4 | 5 | 2 | 4 | 3 | 7 | 10 | N | 10 | N | 10 |
| G2/M | 17 | 19 | 16 | 17 | 18 | 17 | 34 | 40 | N | 39 | N | 40 | |
| G1 | 70 | 61 | 66 | 66 | 61 | 64 | 60 | 49 | N | 50 | N | 54 | |
| 48 | S | 3 | 6 | 5 | 5 | 5 | 2 | 6 | 8 | N | 7 | N | 6 |
| G2/M | 17 | 19 | 18 | 18 | 20 | 20 | 29 | 37 | N | 38 | N | 35 | |
| G1 | 70 | 55 | 68 | 71 | 62 | 68 | 60 | 53 | N | 52 | N | 52 | |
| 72 | S | 2 | 6 | 5 | 3 | 4 | 4 | 6 | 7 | N | 5 | N | 6 |
| G2/M | 17 | 20 | 16 | 16 | 19 | 17 | 28 | 34 | N | 37 | N | 35 | |
LNCaP and PC-3 cells were starved in serum-free medium for 24 hr, followed by stimulation with fresh medium supplemented with 10% FBS and 1 nM DHT in the presence or absence of 10 or 20 uM of DL3 or Bic. The cells were sampled for FACS analysis on 24, 48, or 72 hr. Data shown were from one representative experiment of 7. N = not done.