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. Author manuscript; available in PMC: 2011 Nov 26.
Published in final edited form as: Neurosci Lett. 2010 Sep 19;485(3):233–236. doi: 10.1016/j.neulet.2010.09.019

Figure 3. Effects of NADPH oxidase and glutamate/cystine antiporter inhibitors on Tat-induced regulation of Xc antiporter expression in cultured primary microglia.

Figure 3

Microglia were treated with the NADPH oxidase inhibitor apocynin (200 μM) or the xc− antiporter inhibitor (S)-4-carboxyphenylglycine (4-CPG, 50 μM) in the presence or absence of 100 nM Tat-1-72. xCT mRNA levels were measured by real time PCR after 6 hours. Data indicate the level of xCT mRNA relative to 18S rRNA in each sample, and are means and SEM of 8–12 samples per group compiled from 4 separate experiments. Data were analyzed by 1-way ANOVA, and *** indicates statistically significant (p<0.001) increases in xCT mRNA expression in cells treated with Tat (alone or in the presence of apocynin or 4-CPG) as compared to control cells.