Table 4.

Median (± SEM) Percent False Negative Detection Rates for the Identification of Insulin Secretory Bursts Using the Two Analytical Methods AutoDecon and Cluster.

Sampling duration (min):		30		60		120
Analytical method:		AutoDecon	Cluster	AutoDecon	Cluster	AutoDecon	Cluster
Sampling frequency	Replicates
Every 30 seconds	Singlicates	10.0 ± 0.52	42.2 ± 0.69	7.3 ± 0.35	39.3 ± 0.50	7.1 ± 0.34	39.0 ± 0.38
	Duplicates	5.9 ± 0.39	36.6 ± 0.68	3.5 ± 0.24	33.2 ± 0.50	3.4 ± 0.21	34.2 ± 0.40
	Triplicates	3.7 ± 0.30	23.8 ± 0.58	2.3 ± 0.20	20.2 ± 0.43	1.9 ± 0.15	18.8 ± 0.33
Every 1 minute	Singlicates	25.8 ± 0.85	52.6 ± 0.72	19.8 ± 0.65	49.6 ± 0.51	22.5 ± 0.67	51.2 ± 0.42
	Duplicates	16.6 ± 0.75	47.5 ± 0.70	11.4 ± 0.54	22.9 ± 0.42	12.5 ± 0.55	43.0 ± 0.46
	Triplicates	11.2 ± 0.63	35.4 ± 0.68	8.1 ± 0.48	31.2 ± 0.49	9.3 ± 0.50	29.8 ± 0.41
Every 2 minutes	Singlicates	64.4 ± 0.91	63.0 ± 0.71	57.2 ± 0.74	59.9 ± 0.48	55.1 ± 0.66	61.1 ± 0.38
	Duplicates	63.7 ± 0.91	58.2 ± 0.72	46.8 ± 0.84	53.2 ± 0.49	46.7 ± 0.72	53.2 ± 0.42
	Triplicates	57.6 ± 1.00	52.6 ± 0.69	40.8 ± 0.85	46.3 ± 0.48	41.6 ± 0.74	44.8 ± 0.38