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. 2010 Sep 1;4(5):1164–1173. doi: 10.1177/193229681000400515

Table 1.

High-Performance Liquid Chromatography: Conditions for Profiling Serum and Urine Samplesa

Column Luna phenyl-hexyl 3-μm packing, 150 × 3.0 mm ID
Guard cartridge Phenyl, 3 μm, 4 × 2 mm ID
Mobile phases A 1000:1 (v/v) water:TFA B 900:100:1 (v/v) MeOH:THF:TFA
Mobile phase gradient Time (min) %A %B
0 95 5
35 65 35
37 5 95
40 5 95
41 95 5
Flow rate 0.300 ml/min
a

ID, inside diameter; TFA, trifluoroacetic acid; MeOH, methanol; THF, tetrahydrofuran.