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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Virus Res. 2010 Jun 23;155(1):20–27. doi: 10.1016/j.virusres.2010.06.015

Table 1.

Primers used for real-time PCR and evaluation of standard curves obtained with each primer pair

ORF Primer name Primer sequence (5′ to 3′) Amplicon sizea qPCR standard curve resultsb
R2 slope PCR % efficiency
1 MdvO1F1714
qMdvO1R1882
ATTTCCGCCACACCATACAT
ATTGTTGGGCATCGAGTTGT
169 bp (1714–1882) 0.999 −3.309 100.6
10 MdvO10F133
qMdvO10R7964
CGCAATTGTTCCATGTATCG
GAAGGAGGGCGGTATGGTA
165 bp (8128–7964) 1.000 −3.299 101.0
47 SGHVenvF1
qSGHVenvR1
ACCTACGGTCTGGATATTCGTG
ATAAAGACCCATGCCTGTGC
149 bp (49471–49619) 0.999 −3.242 103.4
86 qMdvO88(86)F94757
MdvO88(86)R767
TGCCATCAAGTGTCTGGAAG
ACAAAAATCTGTCGGGCATC
180 bp (94757–94936) 0.998 −3.187 106.0
106 MdvO108(106)F110
qMdvO108(106)R119549
CGGCAGCAACCTTATTCATT
ACGACGACATCATTTGACGA
130 bp (119420–119549) 0.999 −3.275 102.0
Reference Mdv28sFDQ656974
qMdv28sRDQ656974
GTTAAGCCCGATGAACCTGA
TTGAATAAACTTTCGCCATCG
158 bp N/A N/A N/A
a

Numbers in parentheses indicate the position of the amplicon in the MdSGHV genome.

b

N/A = not applicable.