Table 1.

Primers used for real-time PCR and evaluation of standard curves obtained with each primer pair

ORF	Primer name	Primer sequence (5′ to 3′)	Amplicon sizea	qPCR standard curve resultsb
				R2	slope	PCR % efficiency
1	MdvO1F1714 qMdvO1R1882	ATTTCCGCCACACCATACAT ATTGTTGGGCATCGAGTTGT	169 bp (1714–1882)	0.999	−3.309	100.6
10	MdvO10F133 qMdvO10R7964	CGCAATTGTTCCATGTATCG GAAGGAGGGCGGTATGGTA	165 bp (8128–7964)	1.000	−3.299	101.0
47	SGHVenvF1 qSGHVenvR1	ACCTACGGTCTGGATATTCGTG ATAAAGACCCATGCCTGTGC	149 bp (49471–49619)	0.999	−3.242	103.4
86	qMdvO88(86)F94757 MdvO88(86)R767	TGCCATCAAGTGTCTGGAAG ACAAAAATCTGTCGGGCATC	180 bp (94757–94936)	0.998	−3.187	106.0
106	MdvO108(106)F110 qMdvO108(106)R119549	CGGCAGCAACCTTATTCATT ACGACGACATCATTTGACGA	130 bp (119420–119549)	0.999	−3.275	102.0
Reference	Mdv28sFDQ656974 qMdv28sRDQ656974	GTTAAGCCCGATGAACCTGA TTGAATAAACTTTCGCCATCG	158 bp	N/A	N/A	N/A

^aNumbers in parentheses indicate the position of the amplicon in the MdSGHV genome.

^bN/A = not applicable.