Table 2.
Mean (±SE) MdSGHV genome copy numbers (times 103) detected by real-time PCR in 50 ng of DNA isolated from different tissues of house flies at 72 h post-infectiona
| Tissue | ORF 1 | ORF 10 | ORF 47 | ORF 86 | ORF 106 | all ORFsb |
|---|---|---|---|---|---|---|
| Salivary glands | 319,667 (27,449) | 246,667 (14,310) | 368,667 (24,633) | 616,333 (84,294) | 362,667 (39,976) | 382,800 (37,454)a |
| Midgut | 172,967 (61,687) | 141,800 (48,258) | 205,867 (72,526) | 307,400 (125,758) | 186,133 (70,234) | 202,833 (33,832)b |
| Fat body | 147,867 (38,875) | 113,600 (26,801) | 159,667 (45,322) | 247,000 (65,126) | 148,133 (38,498) | 163,253 (20,692)b |
| Brain | 40,100 (2,318) | 39,967 (786) | 56,467 (273) | 73,000 (2,196) | 47,667 (1,719) | 51,440 (3,364)c |
| Air sacs | no data | 23,484 (16,090) | 32,878 (23,169) | 41,131 (29,899) | 26,346 (18,921) | 30,960 (9,862)d |
| Ovaries | 24,567 (884) | 20,033 (1,033) | 25,867 (899) | 32,167 (1,517) | 22,733 (581) | 25,073 (1,151)d |
| Crop | 877 (503) | 1,601 (1,114) | 2,213 (1,093) | 997 (796) | 1,778 (911) | 1,493 (369)e |
| Hemolymph | 1,980 (n/a) | 620 (620) | 709 (701) | 771 (770) | 696 (692) | 798 (291)e |
a
Means are based on three replicate samples per tissue. Between five and 50 flies were used to obtain each pooled sample (see methods).
b
Mean copy numbers followed by different letters are significantly different (P < 0.05, SNK)