Table 3.
Intensity of bandsa detected on ethidium bromide stained agarose gels after one-step reverse transcriptase PCR with 50 ng of DNase-treated RNA isolated from different samples at 72 h post-infection
| ORF | Viremic salivary glands (SG) | Viremic abdomen w/o SG | Healthy abdomen with SG | ||||||
|---|---|---|---|---|---|---|---|---|---|
| sample 1 | sample 2 | sample 3 | sample 1 | sample 2 | sample 3 | sample 1 | sample 2 | sample 3 | |
| 1 | +++ | +++ | +++++ | − | + | ++++ | − | − | − |
| 10 | ++++ | ++++ | +++++ | ++ | +++ | ++++ | − | − | − |
| 13 | ++++ | ++ | +++++ | − | +++b | +++ | − | − | − |
| 29 | +++++ | + | ++++ | +++ | ++b | ++++c | − | − | − |
| 45 | +++ | ++++ | ++++ | ++++b | +++ | ++++c | − | − | − |
| 47 | +++++ | +++++ | +++++ | + | +++ | ++++ | − | − | − |
| 62 | ++++ | ++++ | ++++ | +++ | +++ | +++ | − | − | − |
| 84 | +++++ | +++ | +++ | +++++c | ++ | ++ | − | − | − |
| 86 | ++++ | ++++ | ++++ | ++++c | ++++c | ++++c | − | − | − |
| 106 | +++ | +++ | +++ | +++c | ++ | +++c | − | − | − |
| Reference | +++++ | +++++ | +++++ | +++++ | +++++ | +++++ | +++++ | +++++ | +++++ |
a
Visually compared and related to the intensity of the reference gene band.
b
Band intensity was higher than in the corresponding salivary gland sample.
c
Band intensity was equal to the corresponding salivary gland sample.