Table 5.
Tissue | ORF1 | ORF10 | ORF47 | ORF86 | ORF106 |
---|---|---|---|---|---|
Salivary glands | 1 | 1 | 1 | 1 | 1 |
Midgut | −77 | −55 | −47 | −63 | −27 |
Ovaries | −112 | −105 | −105 | −102 | −42 |
Fat body | −7 | −23 | −22 | −18 | −4 |
Crop | −15 | −19 | −17 | −29 | −10 |
Air sacs | −4 | −11 | −8 | −9 | −2 |
Brain | −10 | −11 | −29 | −30 | −5 |
Hemolymph | −174 | −201 | −140 | −169 | −109 |
For each ORF normalized threshold cycle values obtained from Table 4 were related to the threshold cycle in salivary gland samples by using the 2−ΔΔCt method as described in Pfaffl (2001).