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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Cell Signal. 2010 Aug 21;23(1):99–104. doi: 10.1016/j.cellsig.2010.08.008

Fig. 2.

Fig. 2

Effect of COX-2 on fibronectin gene expression. (A) Immunoblotting assay for COX-2 expression in THMC infected with AdCOX-2 WT, AdCOX-2G533A and AdCOX-2G533L. β-Actin was used for protein loading control. TCL: total cell lysates. IB: immunoblotting (B) Luciferase assay with the fibronectin promoter in THMC infected with AdCOX-2 WT, AdCOX-2G533A and AdCOX-2G533L. * P < 0.01, ** P < 0.05, *** P < 0.05 by two-tailed Student t-test. (C) Luciferase assay with the fibronectin promoter in THMC under PGE2 stimulation. * P = 0.0001, ** P < 0.001, and *** P < 0.005 by two-tailed Student t-test. All data in B and C are means ± s.e.m from at least three separate experiments, each performed in duplicate.