Fig. 4.
Effect of Rac1 on COX-2mediated fibronectin promoter activity. (A) Constitutive active Rac1, Rac1Q63E, has no effects on COX-2-mediated fibronectin promoter activity. * P < 0.005, ** P = 0.8170 by two-tailed Student t-test. (B) Immunoblotting assay for GFP tagged Rac1Q63E expression in THMC infected with AdCOX-2 WT. β-Actin was used for protein loading control. (C) Immunoblotting demonstrated that ELMO1WT and ET625 have no effects on endogenous COX-2 expression. Data are representative of three independent experiments. All data in A are means ± s.e.m from at least three separate experiments, each performed in duplicate.