Figure 6.
RT-PCR analysis of mESC differentiation on “combinatorial arrays” where different GF types were printed on the same glass substrate. These experiments were performed to assess differences in stem cell differentiation on simple surfaces providing one stimulus vs. complex surfaces providing multiple GF signals. GF mix1 and GF mix2 were once again optimal inducing greatest loss of pluripotency gene Oct 4(A), transient expression of endoderm gene Sox17 (B) and upregulation of hepatic markers AFP and albumin (C).