Table 1.
Sorafenib (ng/mL) | Sorafenib N-oxide (ng/mL) | |||||||
---|---|---|---|---|---|---|---|---|
150 | 800 | 8000 | 80000 (1:10 dilution) |
30 | 200 | 2000 | 20000 (1:10 dilution) |
|
Accuracy | ||||||||
Mean (ng/mL) | 147 | 771 | 8088 | 80161 | 28.5 | 190 | 2103 | 20929 |
SDa | 2.2 | 8.9 | 145 | 2509 | 1.8 | 12.5 | 157 | 1367 |
DEVb (%) | −2.0 | −3.6 | 1.1 | 0.2 | −5.1 | −5.3 | 5.1 | 4.6 |
Precision | ||||||||
Within-run (%) | 1.2 | 0.9 | 1.6 | 0.2 | 6.5 | 6.9 | 6.2 | 0.5 |
Between-run (%) | 1.1 | 0.8 | 1.0 | 0.3 | * | * | 4.6 | 0.5 |
SD: standard deviation;
DEV: deviation from the nominal value;
No significant variation was observed as a result of performing the assay in different runs.