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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Eur J Neurosci. 2010 Sep 30;32(8):1278–1288. doi: 10.1111/j.1460-9568.2010.07402.x

Figure 4. Morphine does not increase the levels of FHC in MOR−/− mice.

Figure 4

(a, b) WT and MOR−/− mice were injected with saline (1 ml/kg) or morphine (20 mg/kg, 1 injection) and sacrificed after 30 min or 6 hr. Tissue homogenates (cortices and hippocampi) were run on a 10% SDS-PAGE followed by immunoblotting using an FHC-specific antibody. The bar graph shows FHC band density normalized to actin and represented with respect to the control (mean ± SEM from 3 separate immunoblots using a total of 3 animals/group); in WT animals FHC band density in the morphine-pretreated (6hr) mice is significantly higher than in the control (F2,15= 9.539, * P < 0.005). (c) Comparison between the basal levels of FHC in WT and MOR−/− mice: Cortical/hippocampal tissue homogenates prepared from MOR−/− mice showed no differences in FHC levels compared to WT (P > 0.05, n=6 animals/each group, i.e. WT and MOR−/−).