(a) After blotting for CXCR4 and KOR, membranes were stripped and reprobed for DOR to confirm equal loading (IP in brain homogenates: t6= 3.387, * P < 0.01, Student’s t-test, n=4 animals/group; IP in glia cultures: t6= 6.274, ** P < 0.005, Student’s t-test, n=4 glial cultures/group). (b) After blotting for DOR and KOR, membranes were stripped and reprobed for CXCR4 to confirm equal loading (IP in brain homogenates: t4= 19.35, ***P < 0.0001, Student’s t-test, n=3 animals/group; IP in glia cultures: t4= 7.771, *P < 0.005, Student’s t-test, n=3 glial cultures/group).