A, left panel, control IK evoked by a 0 mV/30 ms pulse in 40Ko/Nai solutions (see Methods). Right panel, IK left after the immersion of the cell in, 0 K+ solutions (Nao/Nai) for 5 min, with the channels maintained closed at the HP of −80 mV during the exposure to 0 K+ (not shown, indicated by the arrow). B, comparison of the IK remaining either after 5 min exposure to 0 K+, as in A, or after a 25 min recording with K+ present on only one side of the membrane, as indicated.