Fig. 5.

HspB2 inhibits apoptosis downstream of Bid activation. a Whole cell lysates of MDA-MB-231 cells stably expressing HspB2 or empty were subjected to western analysis and probed for HspB2 and tubulin expression. b MDA-MB-231 breast cancer cells stably expressing HspB2 or empty vector were transiently co-transfected with pEGFPN1 and increasing amounts of or pCMV5a-tBid or pCMV5a vector. The percentage of GFP-positive cells with apoptotic nuclei was determined. The data represent the mean ±SEM of three independent experiments (*P < 0.05 versus empty vector at each time point)