Figure 1.

AAV vector transduction frequencies in normal human keratinocytes and HT1080 cells. The titer of an AAV vector containing the human placental alkaline phosphatase gene and packaged in capsids from either serotype 2 or serotype 6 AAV isolates was determined by quantifying probe hybridization signals on alkaline Southern blots. An equal number of genome containing particles was added to cultures of normal human keratinocytes (NHK) or HT1080 cells in parallel. Cells were stained for alkaline phosphatase activity 3 days after infection and foci counted to determine vector titers on each cell type. AAV, adeno-associated virus; ffu, focus-forming unit.