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. 2010 Jun 22;18(9):1667–1674. doi: 10.1038/mt.2010.126

Figure 3.

Figure 3

Fluorescence microscopic analysis of Accell Red siRNA distribution in mouse footpad skin. Transgenic CBL/hMGFP mouse footpads were treated with two (3 × 5) PADs loaded with Accell Red (DY-547-labeled) nontargeting siRNA. Mice were sacrificed at (a,b) 1.5, (c,d) 6, and (e) 24 hours after PAD application and footpad skin sections removed for analysis. Accell Red siRNA (red fluorescence, middle panels) distributes through dermis (d) and epidermis (ep). With this needle design and length, the red fluorescence signal is detected in the basal (b) and spinosum (s) layers at (a,b) 1.5 hours and reaches the granular layer (g) and stratum corneum (sc) at (c,d) 6 hours. Sections were stained with DAPI to visualize nuclei (right panel). Brightfield fluorescence overlay (left panel). Bar = 20 µm. CBL, click beetle luciferase; DAPI, 4,6-diamidino-2-phenylindole; hMGFP, humanized Montastrea green fluorescent protein; PAD, protrusion array device; siRNA, small interfering RNA.