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. 2010 Jun 22;18(9):1667–1674. doi: 10.1038/mt.2010.126

Figure 5.

Figure 5

Analysis of fLuc reporter gene expression following PAD-mediated delivery of expression plasmids to mouse skin. (a) Ear delivery. The ear on the right was treated with a PAD loaded with ~12 ng/microneedle of pGL3-CMV-Luc plasmid (12 microneedles). The ear on the left was treated with the delivery device loaded with PBS vehicle alone. PADs were inserted into the ear for 20 minutes. After 24 hours, luciferase expression was determined following IP luciferin injection by whole animal imaging using the Xenogen IVIS 200 in vivo system (red is the highest expression level, blue lowest). (b) Footpad delivery. Right footpads were treated with PADs (12 microneedles) loaded with luciferase expression plasmid for two consecutive days and analyzed as described above. Left footpads were treated with PADs loaded with PBS vehicle alone (control). fLuc, firefly luciferase; IP, intraperitoneal; PAD, protrusion array device; PBS, phosphate-buffered saline.